Abstract
BackgroundS. aureus is one of the major etiological agents causing bovine subclinical mastitis. The regulatory effects of H3K27me3 on gene expression in subclinical S. aureus mastitis cows are unknown. This study aimed to profile genome-wide transcriptional changes regulated by H3K27me3 in bovine lymphocytes applied in subclinical S. aureus mastitis cows and healthy controls.ResultsA total of 61 differentially expressed genes (DEGs) were detected in subclinical S. aureus mastitis cows compared to the healthy controls, of which 25 DEGs are up-regulated and the rest are down-regulated genes in subclinical S.aureus mastitis cows. The up-regulated genes are mainly involved in the Jak-STAT signaling pathway, cytokine-cytokine receptor interaction, and T cell receptor-signaling pathway, while the down-regulated genes are related to metabolism pathways. Combination analysis of histone methylation and gene expression revealed that H3K27 trimethylation levels in silent genes were higher in subclinical S. aureus mastitis cattle than in healthy cows. The key regions of H3K27me3 target genes related to subclinical S. aureus mastitis were the upstream 2 kb regions of the DEGs relative to transcription start site (TSS).ConclusionsThe current study provides a novel insight into the interaction between S. aureus and lymphocytes in lactating cows by histone H3 methylation regulation. The differentially expressed genes in bovine lymphocytes regulated by H3K27me3 on upstream 2 kb regions (IL10, PTX3 and etc.) may relate to S. aureus mastitis susceptibility and could be considered as key candidate genes for anti- S. aureus mastitis study and breeding.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-016-2947-0) contains supplementary material, which is available to authorized users.
Highlights
S. aureus is one of the major etiological agents causing bovine subclinical mastitis
We selected seventeen Holstein cows as candidate subclinical mastitis dairy cattle based on their dairy herd improvement (DHI) records throughout the whole lactation period
The result of bacteria culture showed as suspected that S. aureus (Fig. 1b) and gram stain was positive (Fig. 1c) for mastitis cattle while the number of bacteria detected was quite small for healthy cows (Fig. 1d)
Summary
S. aureus is one of the major etiological agents causing bovine subclinical mastitis. Subclinical mastitis is one of the major challenging diseases to the modern dairy industry [1]. It affects both quantity and quality of milk [2,3,4,5]. The widespread prevalence of S. aureus in the environment suggests that it is an unlikely the pathogen to be eradicated [12, 13]. He et al BMC Genomics (2016) 17:565 deciphering the interaction between cows and S. aureus infection would make controlling this complex disease in dairy cattle become more practical. H3K27me was found to play a vital role in development, imprinting, carcinogenesis, and inflammatory diseases [17]
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