Abstract
This entry reviews the types of physical map that can be constructed, the methods used to construct them and their most likely current use. In particular, the emphasis is on the construction of genomic restriction maps and ordering overlapping libraries using top-down mapping approaches to enable de novo genome sequencing and for gap filling in sequencing projects. Also explored is the use of these approaches in functional studies. Keywords: Alu Repeat; Bacterial Artificial Chromosome (BAC); Cloneless Library; Comparative Genomic Hybridization (CGH); Expressed Sequence Tag (EST); Fluorescence In Situ Hybridization (FISH); Inter-Alu PCR; Jumping Libraries; Kpn Repeat; Linking Libraries; Locus; Long Interspersed Repeat Elements (LINEs); Multiplex Analysis; P1-derived Artificial Chromosomes (PAC); Polonies; Polymerase Chain Reaction (PCR); Polymorphism Link-up; Pulsed Field Gel (PFG) Electrophoresis; Radiation Hybrid Maps; RARE (RecA-assisted Restriction Endonuclease) Cleavage; Retroposons; Sequence-tagged Restriction Site (STAR); Sequence-tagged Site (STS); Short Interspersed Repeat Elements (SINEs); Yeast Artificial Chromosome (YAC)
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
