Whole fungal elicitors boost paclitaxel biosynthesis induction in Corylus avellana cell culture.

Mina Salehi,Siamak Farhadi,Ahmad Moieni,Naser Safaie,Christophe Hano

doi:10.1371/journal.pone.0236191

Abstract

Paclitaxel is an effective natural-source chemotherapeutic agent commonly applied to treat a vast range of cancers. In vitro Corylus avellana culture has been reported as a promising and inexpensive system for paclitaxel production. Fungal elicitors have been made known as the most efficient strategy for the biosynthesis induction of secondary metabolites in plant in vitro culture. In this research, C. avellana cell suspension culture (CSC) was exposed to cell extract (CE) and culture filtrate (CF) derived from Camarosporomyces flavigenus, either individually or combined treatment, in mid and late log phase. There is no report on the use of whole fungal elicitors (the combined treatment of CE and CF) for the elicitation of secondary metabolite biosynthesis in plant in vitro culture. The combined treatment of CE and CF significantly led to more paclitaxel biosynthesis and secretion than the individual use of them. Also, multivariate statistical approaches including stepwise regression (SR), ordinary least squares regression (OLSR), principal component regression (PCR) and partial least squares regression (PLSR) were used to model and predict paclitaxel biosynthesis and secretion. Based on value account for (VAF), root mean square error (RMSE), coefficient of determination (R2), mean absolute percentage error (MAPE) and relative percent difference (RPD) can be concluded that mentioned regression models effectively worked only for modeling and predicting extracellular paclitaxel portion in C. avellana cell culture.

Highlights

Paclitaxel is a well-known chemotherapeutic agent widely applied as a therapy for various types of cancers [1], and this is used as a treatment for non-cancerous human diseases [2]
dry weight (DW) of the cells in C. avellana cell suspension culture (CSC) exposed with E100/0, E75/25, E50/50, E25/75 and E0/100 derived from C. flavigenus in mid and late log phase were measured as cell growth
Analysis of variance (ANOVA) demonstrated that the main effects of factors “elicitor type, concentration level and elicitor adding day” and their reciprocal interactions on DW were not significant (Table 2), suggesting that fungal elicitors, regardless of its adding day and concentration levels, did not have an impact on cell growth in C. avellana CSC (Fig 1). It displays adaptability of C. avellana cells to elicitors derived from C. flavigenus, in view of the fact that strain HEF17 is a symbiont of this plant

Summary

Introduction

Paclitaxel is a well-known chemotherapeutic agent widely applied as a therapy for various types of cancers [1], and this is used as a treatment for non-cancerous human diseases [2]. Paclitaxel as a cytoskeletal drug arrests the proliferation of tumor cells following stabilizing the microtubules. This valuable metabolite blocks cell cycle in G0/G1 and G2/M phases by interaction with tubulin [3]. This fantastic diterpene alkaloid, paclitaxel, was originally found in the bark of Taxus brevifolia tree [4].

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