Whole‐Cell Voltage Clamp Recording

Abstract

This unit describes the use of whole-cell voltage clamping to study voltage-gated channels. Stepwise changes in voltage produced by this technique cause channels to interconvert between different states, and these transitions are monitored as changes in membrane current. The time course of this redistribution of states contains a great deal of information about the mechanism of channel gating. Furthermore, the voltage clamp can be used to activate different populations of channels selectively. In this way, a specific channel targeted by biological or pharmacological manipulations can often be identified and studied in detail. This technique is also readily adapted to the study of ligand-gated channels, synaptic potentials, and exocytosis.