Abstract
Objectives: The aim of the present study was to develop a simple, quick and cheap method to process whole-blood samples for the molecular techniques polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) without the use of expensive reagents or sophisticated machines. Materials and Methods: Venous whole-blood samples were collected from 40 individuals. The samples were frozen at –80°C, and then rapidly thawed at 37°C. Each sample was incubated with distilled water, then boiled in a microwave and centrifuged. The supernatant was taken directly for PCR and RFLP. For comparison, PCR and RFLP were performed on DNA purified from the same samples using the phenol-chloroform method and two commercial DNA extraction kits. Results: PCR/RFLP results using the presented method were qualitatively similar to those obtained by DNA extracted using the other three methods. Conclusion: The presented method proved to be a simpler and cheaper way of processing whole-blood samples for PCR and RFLP analyses.
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