Abstract
Whitening cosmetics market has a bright future, and pure natural whitening products of traditional Chinese medicine have always been a research hotspot. In this research, the whitening active ingredient of Chinese medicine Trichosanthes pulp was isolated and purified for the first time, and its whitening mechanism was clarified. Chromatographic methods such as silica gel, ODS, and HPLC were used to isolate and purify them. B16 cells were used to measure the antioxidant activity, tyrosinase activity, and melanin removal activity. A total of 20 compounds were isolated, including p-hydroxybenzaldehyde (1), salicylic acid (2), vanillic acid (3), isovanillic acid (4), protocatechuate (5), trans-cinnamic acid (6), 4-coumaric acid (7), trans-ferulic acid (8), drechslerol-B (9), cyclotucanol 3-palmitate (10), 5-acetoxymethyl-2-furaldehyde (11), 5-hydroxymethylfurfural (12), diosmetin (13), apigenin (14), chrysoeriol (15), luteolin (16), 4′-hydroxyscutellarin (17), quercetin (18), 3′,5-dihydroxy-7-(β-D-glucopyranosyloxy)-4′-methoxyflavone (19), and cofloxacin-7-O-β-D-glucoside (20). Among them, compounds 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, and 20 have good antioxidant repairing effects; compounds 3, 4, 5, 6, and 7 have high black inhibition; compounds 1, 2, 3, 4, 5, 6, 7, 8, 11, 12, 13, 14, 15, 16, 17, 18, 19, and 20 have obvious tyrosine acidase inhibitory activity. The results laid foundation for the further development and utilization of Trichosanthes pulp resources and also provide a basis for the development of natural whitening cosmetics.
Highlights
Trichosanthes kirilowii Maxim. is a perennial climbing herb of the Cucurbitaceae family
Reports on Trichosanthes pulp are mainly focused on the content comparison of the sugar, amino acids, vitamins, and other nutrients [1,2,3,4]. ere is no report on the chemical composition of Trichosanthes pulp. erefore, it is necessary to study the chemical constituents of the pulp systematically
Many classical Chinese medicine books or materia medica has recorded that Trichosanthes pulp has the function of whitening and wrinkle removing effects [5,6,7]. e previous results of our research group show that the water extract of Trichosanthes pulp had higher tyrosinase inhibitory activity, and the tyrosinase inhibitory rate was about 70%. e common whitening agents vitamin C (VC) and arbutin were used as control substances [8]
Summary
Trichosanthes kirilowii Maxim. is a perennial climbing herb of the Cucurbitaceae family. It was preliminarily proved that the extract of Trichosanthes pulp had a certain whitening effects by inhibiting the activity of tyrosinase. Erefore, it is of great significance to study the chemical constituents and whitening mechanism of Trichosanthes pulp. Erefore, the reduction with antioxidant effects can interact with the copper atom on the active site of tyrosinase or reduce the intermediate in the process of melanin synthesis to inhibit the formation of melanin [10, 11]. Cell culture technology is used to determine the effect of whitening active ingredients on tyrosinase activity and melanin production in melanocytes in vitro. Erefore, B16 cells were used to measure the antioxidant activity, tyrosinase activity, and melanin removal activity, and the whitening active components and the related mechanism were determined preliminarily. Since it is very difficult to culture human primary skin melanoma cells, the mouse model can be used as a test cell for the determination of whitening active ingredients. erefore, B16 cells were used to measure the antioxidant activity, tyrosinase activity, and melanin removal activity, and the whitening active components and the related mechanism were determined preliminarily. e results laid foundation for the further development and utilization of Trichosanthes pulp resources and provide a basis for the development of natural whitening cosmetics
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