Abstract
The aim of this study was to evaluate the effect of whey protein concentrate (WPC-80) on glycoconjugate catabolism, selected markers of oxidative stress and liver inflammation. The experiment was conducted on male Wistar rats (n = 63). The animals from the study group were administered WPC-80 at a dose of 0.3 or 0.5 g/kg body weight for 7, 14 or 21 days, while rats from the control group received only 0.9% NaCl. In liver homogenates, we assayed the activity of N-acetyl-β-D-hexosaminidase (HEX), β-glucuronidase (GLU), β-galactosidase (GAL), α-mannosidase (MAN), α-fucosidase (FUC), as well as the level of reduced glutathione (GSH), malondialdehyde (MDA), interleukin-1β (IL-1β) and transforming growth factor-β1 (TGF-β1). A significantly higher activity of HEX, GLU, MAN and FUC were found in the livers of rats receiving WPC-80 compared to controls. Serum ALT and AST were significantly higher in the animals supplemented with WPC-80 at a dose of 0.5 g/kg body weight for 21 days. In the same group of animals, enhanced level of GSH, MDA, IL-1β and TGF-β1 were also observed. WPC-80 is responsible for intensive remodelling of liver tissue and induction of oxidative stress especially at a dose of 0.5 g/kg body weight.
Highlights
In recent years, there has been an increase in interest in whey [1]
In the liver of rats fed with WPC-80 at a dose of 0.3 g/kg body weight histological studies, we showed that WPC-80 leads to(Figure liver damage with the observed features for 7Indays, damage to single cells were demonstrated
Our study study is is the the first first to to indicate indicate increased increased catabolism catabolism of of liver liver glycoconjugates glycoconjugates in in the the rats rats supplemented whey protein protein concentrate concentrateWPC-80
Summary
There has been an increase in interest in whey [1]. It is a by-product of cheese production and a rich source of exogenous amino acids and biologically active proteins. It has been proven that α-lactalbumin and β-lactoglobulin are the main whey proteins, forming up to 80% of the protein mass [2,3]. Due to its varied composition, whey is commonly supplemented to emaciated patients (e.g., during convalescence or cancer cachexia), children with cow’s milk protein allergy and sportsmen to increase their muscle mass [1,4]. Whey proteins are a valuable source of sulphuric amino acids: cysteine and methionine, crucial for the synthesis of reduced glutathione (GSH) [5]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
