Abstract
Beginning from the birth of RNA to its death, RNA-protein interactions require, in many instances, specific recognition of the RNA target by a protein. Specific recognition underlies mRNA processing, chromosome-end duplication and many developmental events such as early patterning of the Drosophila embryo. Decisions on cell fate during early stages of development regulate the activity, stability and cellular localization of mRNAs. Several experimental strategies to detect RNA-protein interactions have been designed to assess in vivo and in vitro interactions. Recently a variation of the widely used two hybrid transcriptional transactivation assay for detection of protein-protein interactions has been adapted to explore functional RNA-protein interactions in vivo. In this genetic assay, termed the three-hybrid assay, the budding yeast is used as the biological system, and the assay is based on interactions between two fusion proteins and a hybrid RNA, which are used to activate transcription of reporter genes (SenGupta et al 1996).
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