Abstract
Nowadays, genomic sequencing data are being generated at a speed that has outstripped the pace of annotation. Loss-of-function CRISPR screens have been used as a high-throughput approach to determine the functional relevance of individual genes in a specific phenotype by ablating gene expression on a genome-wide scale and studying the resulting phenotype alterations.1,2 This is made possible by combining the CRISPR-Cas9 gene editing system with libraries of single guide RNAs (sgRNAs), which are ideally designed to target every gene in the genome.
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