Abstract
Wheat gluten hydrolysates (WGH) and their fractions (0%, 20% and 40% ethanol fractions) obtained on XAD-16 were used to examine their effects on multiple stress tolerance and ethanol fermentation performances of yeast during very high-gravity (VHG) fermentation. Results showed that WGH and their fractions supplementations increased significantly osmotic/ethanol stress tolerance, growth and vitality of yeast cells. Supplementation with 40% ethanol fraction resulted in 46.54% and 35.01% of increase in cell membrane integrity, and 42.59% and 21.79% of reduction in intracellular ROS level under osmotic and ethanol stress conditions, respectively. Moreover, WGH and their fractions supplementations also increased physiological activity and ethanol yield of yeast cells under VHG fermentation, and 40% ethanol fraction supplementation caused the maximum biomass and ethanol production during VHG fermentation. These results suggested that WGH supplementation was an efficient and convenient method for improvement of yeast cells with enhanced multiple stress tolerance and ethanol fermentation performances.
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