Wheat and barley differently affect porcine intestinal microbiota.

Abstract

Diet influences the porcine intestinal microbial ecosystem. Barrows were fitted with ileal T-cannulas to compare short-term effects of eight different wheat or barley genotypes and period-to-period effects on seven bacterial groups in ileal digesta and faeces by qPCR. Within genotypes of wheat and barley, there was no difference (P > 0.05) in contents of analysed NSP, yet cereal types differed (P < 0.001) except for soluble arabinoxylans. Genotypes showed no effect on bacterial gene copy numbers. In ileal digesta of barley- compared to wheat-fed pigs, log10 copy numbers were lower (P < 0.05) for total eubacteria (9.6-9.8), Bacteroides-Prevotella-Porphyromonas (6.5-6.8), Clostridium cluster IV (6.7-6.9), and Roseburia spp. (6.6-7.2), while higher copy numbers were found for Lactobacillus spp. (9.4-8.8). Enterobacteriaceae (7.0-7.8) and Bifidobacterium spp. (7.0-7.7) were lower (P < 0.001) in faeces of barley compared to wheat-fed pigs. Ileal eubacteria, Clostridium cluster IV and Roseburia spp. linearly increased from period 1 to 8 for both cereals (P < 0.05). Wheat and barley differently influence microbial composition particularly in the small intestine, with barley increasing the Lactobacillus spp.:Enterobacteriaceae ratio, underlining its potential to beneficially manipulate the intestinal microbial ecosystem.