What is the Best Degree of Hyaluronic Acid Crosslinking in Increasing Growth Factors Level of Platelet-Rich Fibrin Lysate?

Nora Ariyati,Nurdiana Nurdiana,Kusworini Handono,Yohanes Widodo Wirohadidjojo

doi:10.46582/jsrm.1501002

Nora Ariyati, Nurdiana Nurdiana + Show 2 more

Open Access

https://doi.org/10.46582/jsrm.1501002

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Abstract

Introduction: Various therapeutic materials such as hyaluronic acid (HA) and platelet-rich fibrin lysate (PRF-L) have been represented to improve chronic fibroblast ulcers and premature aging. HA crosslinking is the most popular dermal filler presently in the therapy of aging skin. With the PRF-L properties that are rich in growth factors (GF), the combination of these materials is expected to produce synergistic and potentiation effects. Objective: This study aimed to determine the effect of various degrees of HA crosslinking on GF levels in PRF-L. Materials and Methods: PRF-L was obtained from PRF of healthy adult venous blood with 72 hours of incubation. HA was taken from preparations with 3 crosslinking degrees of 3%, 4%, and 10%. Measurement of GF levels was performed using sandwich ELISA method. Results: The GF levels released in PRF-L increased with the addition of HA crosslinking. The lower HA crosslinking degree (3%) triggered greater release of GF by PRF-L compared to higher HA crosslinking degree (4% & 10%). Conclusion: Low degree HA crosslinking elevated all measured GF levels in PRF-L. The lower HA crosslinking degree provoked higher release of GF.

Highlights

Various therapeutic materials such as hyaluronic acid (HA) and platelet-rich fibrin lysate (PRF-L) have been represented to improve chronic fibroblast ulcers and premature aging
This study aimed to determine the effect of HA mixture with various degrees of crosslinking on growth factors (GF) levels in PRF-L
PRF-L was a lysate obtained from PRF isolated from volunteer blood with 50% levels dissolved in Dulbecco’s minimal essential medium (DMEM) + fetal bovine serum (FBS) 1% (GibcoTM, Massachusetts, USA)[13]

Summary

Introduction

Various therapeutic materials such as hyaluronic acid (HA) and platelet-rich fibrin lysate (PRF-L) have been represented to improve chronic fibroblast ulcers and premature aging. Objective: This study aimed to determine the effect of various degrees of HA crosslinking on GF levels in PRF-L. Results: The GF levels released in PRF-L increased with the addition of HA crosslinking. Conclusion: Low degree HA crosslinking elevated all measured GF levels in PRF-L. Synthetic materials frequently utilized as fillers of aging skin tissue through injection include hyaluronic acid (HA). HA is able to restore volume and elevate the amount of collagen in the dermis through fibroblast stimulation. This re-volumization causes water retention at various levels, increasing turgor and skin hydration. HA is mere temporary in collagen and elastin volume replacement that continues to disappear and fail to make it revert[1]

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