Abstract
What do we learn from cultures in the omics age? High-throughput sequencing and cultivation of leaf-inhabiting endophytes from beech (Fagus sylvatica L.) revealed complementary community composition but similar correlations with local habitat conditions
Highlights
Fungal endophytes reside in the living tissues of plants without causing visible disease symptoms (e.g. Christian et al 2015)
On the other hand our results demonstrate that the immense body of literature about cultivable endophytic fungi can and should be used for the interpretation of community signals and environmental correlations obtained from high-throughput sequencing (HTS) studies and that cultivation studies should be continued at the highest standards, e.g. when sequencing facilities are not available or if such surveys are expanded into functional aspects with experiments on living isolates
In addition and contrary to HTS data, Hill numbers N2 and N3 were significantly different for cultivation data (Fig. 1B, for statistical details see Table S2 and S3; “Suppl. material 3”)
Summary
Fungal endophytes reside in the living tissues of plants without causing visible disease symptoms (e.g. Christian et al 2015). Particular research interest is given to endophytes of the phyllosphere and other photosynthetic organs due to the enormous availability and environmental importance of leafy habitats (Lindow and Brandl 2003), the complex biochemical processes in these plant tissues and the generally close interconnectivity of leaf mycobiome with their hosts (reviewed in Rodriguez et al 2009, Peršoh 2015). Peršoh 2013, Jumpponen and Brown 2014, Bálint et al 2015, Eusemann et al 2016, Purahong et al 2016). These improvements go side by side with the ever increasing accuracy of reference sequence databases (Nilsson et al 2014, 2015, Abarenkov et al 2016)
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