Abstract
Noncovalent functionalization of single-walled carbon nanotubes (SWCNT) with amphiphilic polymers has significantly advanced the colloidal SWCNT efficacy for biomedical optical sensors. Single-strand DNA (ssDNA), as an amphiphilic biocompatible polymer, has been employed to fabricate ssDNA-wrapped SWCNT complexes, however the correlation between DNA sequence and wrapping affinity is not fully studied. In this research, we evolved and screened high-affinity ssDNA sequences from ~1015 ssDNA (random 30-mer), and analyzed the influence of DNA sequence on the wrapping affinity. Wrapping affinity of specific ssDNA sequence was quantitatively measured by the rate constant when ssDNA at the surface was exchanged to sodium cholate. The wrapping affinity relies on the base composition and k-mer motif. Also, we build a machine learning model to identify the high affinity DNA sequence with >85% accuracy. These results indicate the presence of “sequence motif(s)” to control the wrapping affinity and chemical conformation of ssDNA molecule on the SWCNT surface, which can be useful for developing DNA-assisted carbon nanosensors.
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