Abstract
Filamentous fungi with chitin as a major component of their cell walls produce chitinases at all stages of active growth, i.e. during spore germination, exponential growth and mycelial development. The roles of chitinases in these processes have been investigated by assessing the effects of treatment with the inhibitor, allosamidin. Inhibition has only been observed, however, of spore germination, of cell separation in budding yeasts and of the action of a yeast toxin, and not of apical extension or branching. This may be explained by the finding that chitinase activities in situ have different properties to those in cell homogenates, and appear to be protected from environmental stresses.
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