Abstract
Improvements in the wettability of the Ti6Al4V alloy following CO(2) laser treatment were identified as being due mainly to the increase in surface roughness, surface oxygen content and surface energy of the material. Untreated and mechanically roughened samples had higher amounts of adsorbed albumin and lower amounts of adsorbed fibronectin than CO(2) laser treated samples. Moreover, as the wettability of the Ti6Al4V alloy increased the adsorbed amounts of fibronectin increased, while the adsorbed amounts of albumin decreased--indicating the controllability of the CO(2) laser process. From this finding it is possible to assert that the wettability of the Ti6Al4V alloy was the prime influence on the observed changes in in vitro protein adsorption. Further, the noted considerable change in the polar component of surface energy, [Formula: see text], on the protein adsorption implied that the protein adsorption on the Ti6Al4V alloy was probably due to the polar and chemical interactions. This work has demonstrated that CO(2) laser radiation could be a suitable means to modify the wettability of the Ti6Al4V alloy and thereby manipulate protein adsorption and consequently render the material more bone cell responsive.
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