Abstract
BackgroundEosinophils (EOS) is one of the most important cells involved in the pathogenesis of chronic airway inflammation in asthma, and its apoptosis is part of the mechanisms of asthma. Therefore, this study aimed to observe the effect of Chinese medicine Wentong decoction (WTD) in EOS apoptosis in asthmatic rats. This work also explored the mechanism of WTD regulation in EOS apoptosis and provided a new target for clinical treatment of asthma.MethodsAsthmatic rats induced by ovalbumin were treated with WTD. Lung function of rats in each group was detected, and lung tissue pathology, EOS counts in blood and bronchoalveolar lavage fluid were observed. The degree of the EOS apoptosis in rats was detected. The expression content of interleukin (IL)-5, IL-10, chemokine (C–C motif) ligand 5 (CCL5), granulocyte–macrophage colony-stimulating factor (GM-CSF), transforming growth factor beta 1 (TGF-β1), interferon (IFN)-γ, and other cytokines in rat serum and the genes of Eotaxin mRNA, Fas mRNA, FasL mRNA, Fas/FasL and Bcl-2 mRNA in the lung tissues were determined.ResultsWTD can reduced airway resistance in rat models and improved airway compliance. The pathological changes of lung tissue in WTD group were significantly alleviated, at the same time, WTD could reduce the EOS count in the blood and BALF smears of the asthmatic model rats. Compared with the model group, the apoptosis degree of EOS significantly increased in rats in the WTD group. The expression of IL-5, CCL5, and GM-CSF in the serum and the expression of Eotaxin mRNA, Bcl-2 mRNA in the lung tissues in rats in the WTD group rats decreased. Moreover, the expression of IL-10, TGF-β1, and IFN-γ in the serum and the expression of Fas mRNA, FasL mRNA in the lung tissues in rats in the WTD group rats increased compared with that in rats in the model group.ConclusionsWentong decoction may accelerate EOS apoptosis, reduce asthma inflammation, and alleviate the disease through regulating and controlling the factors related to the anti-apoptosis and pro-apoptosis.
Highlights
Eosinophils (EOS) is one of the most important cells involved in the pathogenesis of chronic airway inflammation in asthma, and its apoptosis is part of the mechanisms of asthma
RNA extraction and real‐time polymerase chain reaction (PCR) We evaluated the effect of Wentong decoction on the expression of Eotaxin mRNA and Fas mRNA in the lung tissues of asthmatic rats by using real-time fluorescent quantitative PCR
The results suggested that Wentong decoction (WTD) may be able to reduce the Detection of EOS apoptosis To evaluate the EOS apoptosis in asthmatic rats and the effect of WTD on the degree of EOS apoptosis, we adopted flow cytometry and transferase dUTP nick end labeling (TUNEL) method, respectively, to determine the EOS apoptosis in the arterial blood and lung tissues of rats (Figs. 5, 6)
Summary
Eosinophils (EOS) is one of the most important cells involved in the pathogenesis of chronic airway inflammation in asthma, and its apoptosis is part of the mechanisms of asthma. Bronchial asthma (BA) is a chronic inflammatory disease of the airway associated with multiple cells (such as eosinophils, mast cells, T-lymphocytes, neutrophils, and airway epithelial cells) and the cellular elements [1]. This inflammation causes the susceptible person to exhibit high-airway reactivity to each kind of motivating factor causing airway constriction, wherein eosinophil (EOS) infiltration of airway leads to airway inflammation and airway pathology change is an important sign of clinical changes of asthma disease [2, 3]. The prevalence rate of adult asthma is 1.2–25.5% [7]
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