Well Plate Integrated Topography Gradient Screening Technology for Studying Cell-Surface Topography Interactions.

Torben A B Van Der Boon,Jan De Boer,Patrick Van Rijn,Daniel E Córdova Galván,Liangliang Yang,Linfeng Li,Qihui Zhou

doi:10.1002/adbi.201900218

Abstract

New high-throughput technologies for cell-material interaction studies provide researchers with powerful tools to speed up research in the field of biomaterial-cell interactions. However, sharing technologies is often difficult due to the necessity of specific knowledge and experiences. Engineered surfaces can elucidate effects of surface topography on cell behavior, which is of critical value for gaining control over cellular processes. Here, the translation of a gradient-based high-throughput cell screening approach for aligned nano/micro topographies interacting with cells is presented. An aligned topography 96-well plate is created by upscaling of highly specific gradient technology. The resulting cell culture dishes are compatible with general laboratory and imaging equipment, and the platform allows for studying cell behavior with regard to adhesion and alignment. The challenge lies in increasing the dimensions of the previous 1 × 1 cm gradient topography substrate, to be able to cover the span of a 96-well plate and translate it into a standardized cell-screening tool. Adhesion experiments of human bone marrow derived mesenchymal stem cells confirm the standardization, compatibility, and usability of the technology. In the process of using multi-system imaging and analysis, it becomes apparent that future challenges need to include universally applied data analysis approaches.

Highlights

IntroductionThe translation of a gradient-based high-throughput cell screening approach for aligned trigger an altered cellular behavior such as a foreign body response.[4,5,6,7] As a result, in ongoing research, the trend is shifting to developing more bio-instructive[8,9] instead of bio-inert materials
Using a gradient means that great care needs to be taken when working with a gradient as it is pivotal to be able to accurately identify where on the surface the analysis is performed, as a small deviation may result in misinterpretation as to what topography is being analyzed
In the development of high-throughput approaches for studying cell–material interactions, we found that the gradients we have developed in the past serve the purpose of these investigations

Summary

Introduction

The translation of a gradient-based high-throughput cell screening approach for aligned trigger an altered cellular behavior such as a foreign body response.[4,5,6,7] As a result, in ongoing research, the trend is shifting to developing more bio-instructive[8,9] instead of bio-inert materials. The challenge lies in increasing the dimensions of the previous 1 × 1 cm gradient topography parameters, such as the material’s chemistry, stiffness, and topography.[10,11,12,13,14,15,16,17,18,19,20] The chemistry and wettability of a surface together dictate the adsorbance of proteins to the surface, which influences cell adhesion.[19,21,22] Identifying how such substrate, to be able to cover the span of a 96-well plate and translate it into material parameters influence cellular a standardized cell-screening tool.

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Conclusion