Abstract
The anaerobic fermentation of glucose and fructose was performed by Actinobacillus succinogenes 130Z in batch mode using three different volume of bioreactors (0.25, 1 and 3 L). The strategy used was the addition of MgCO3 and fumaric acid (FA) as mineral carbon and the precursor of succinic acid, respectively, in the culture media. Kinetics and yields of succinic acid (SA) production in the presence of sugars in a relevant synthetic medium were investigated. Work on the bench scale (3 L) showed the best results when compared to the small anaerobic reactor’s succinic acid yield and productivity after 96 h of fermentation. For an equal mixture of glucose and fructose used as substrate at 0.4 mol L−1 with the addition of FA as enhancer and under proven optimal conditions (pH 6.8, T = 37 °C, anaerobic condition and 1% v/v of biomass), about 0.5 mol L−1 of SA was obtained, while the theoretical production of succinic acid was 0.74 mol L−1. This concentration corresponded to an experimental yield of 0.88 (mol-C SA/mol-C sugars consumed anaerobically) and a volumetric productivity of 0.48 g-SA L−1 h−1. The succinic acid yield and concentration obtained were significant and in the order of those reported in the literature.
Highlights
Published: 31 July 2021Succinic acid (SA), which is a biomolecule, is well-known as a platform chemical and as a highly versatile building block used in a variety of industrial applications namely, surfactants, green solvents and pharmaceutical compounds, because of its linear and saturated structure [1,2]
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MgCO3 was screened to be a key factor in SA production [31] and, it was added to the fermentation medium as a neutralizer and to redirect the metabolic flux at 0.1 mol L−1 concentration
Summary
Succinic acid (SA), which is a biomolecule, is well-known as a platform chemical and as a highly versatile building block used in a variety of industrial applications namely, surfactants, green solvents and pharmaceutical compounds, because of its linear and saturated structure [1,2]. The world is at a critical changeover point as we move ahead into a bio-economy, while simultaneously reducing dependence on finite fossil fuels and leaving behind the petroleum-based economy [7]. According to this scenario, petroleum refineries could be progressively replaced by biorefineries as governments pursued the generation of renewable energy, bio-fuels and bio-derived chemicals [8,9]. The preferable approach for bio-renewable chemicals is the Publisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations
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