マウスのWeil-Felix反応 (抗OXK菌) に関する研究

Abstract

For the purpose to establish the method to classify the Rickettsia orientalis serologically, the possibility to produce OXK antibody in mice infected or immunized with it was tested.After it was found to be possible to produce OXK antibody in mice by immunization with the killed vaccine of Proteus OXK, the following methods were tested to detect OXK antibody in mice. i) Mice were infected with the infective organ emulsion (spleen and liver of mice) through subcutaneus route with and without Freund-type adjuvant. Almost all mice infected could survive during the observed periods of 3 or more weeks, although the control mice died within 2 weeks after the infection of intraperitoneal route with it. ii) Mice were immunized with the killed vaccine made from the infective organ with and without adjuvant.Two strains of R. orientalis, Kato (Niigata, Japan) and Karp (New Guinea), were used in these experiments. These strains had already identified as R. orientalis serologically.By the infection through subcutaneus route, antibody could be detected in mice sera remarkably following inoculation of vaccine combined with adjuvant, but not so remarkably without adjuvant. On the other hand, by the immunization with the killed vaccine, it was found that antibody formation in mice was easy with adjuvant, but very difficult without adjuvant.From these results, it was suggested that it is possible to produce OXK antibody in mice by the immunization of the killed vaccine of R. orientalis with adjuvant, and this method may be applicable to the serological classification of it.