Abstract
Humic acids (HAs) from four soils of different origin (podzoluvisol, phasozem, chernozem and ferralsol) have been fractionated by coupling size exclusion chromatography (SEC) and polyacrylamide gel electrophoresis (PAGE). Preparative quantities of HA fractions (HAFs) with different molecular sizes (MSs) and exactly defined electrophoretic mobility (EM) have been obtained from all the samples. Fractions identically marked had similar EM, independently of HA sources. The weight content, extinction coefficients (ECs), E4/E6 ratios have been studied for all HAFs. EC values and E4/E6 ratios increased as MS decreased within an individual HA sample. The weight content of high MS fraction was the highest in podzoluvisol and ferralsol and the smallest in phasozem and chernozem. On the other hand, chernozem and phasozem HAs contained considerably more of the lower MS fraction than HAs from the podzoluvisol and ferralsol. The knowledge of weight and optical differences between soil humic acids fractionated by coupling SEC-PAGE could be valued for explanation of some physico-chemical properties of soils and influence of soil genesis on humic matter properties.
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