Weather, ultrasonic, cranial and body traits predict insect diet hardness in a Central Mexican bat community

Abstract

Insectivorous bats exhibit food preferences for specific attributes in their prey. Hardness has been defined as an important prey attribute, and in some cases a limiting factor in foraging decisions for smaller compared to larger bat species. The goal of this study was to identify which factors influence the selection of prey hardness in a vespertilionid bat community. We investigated food consumed by bats by analyzing fecal samples obtained from eight bat species coexisting in a mountain ecosystem of central Mexico and correlate non-phylogenetically and phylogenetically prey hardness to weather, bat´s body, cranial and ultrasonic call structure variables. Results showed that diet of vespertilionid bats was mainly represented by Diptera, Neuroptera, Lepidoptera and Coleoptera consumption. The qualitative prey hardness index (From soft 1 to hard 5) ranked bats as: Myotis melanorhinus, Corynorhinus mexicanus, Myotis volans, Myotis californicus (< 3); Myotis velifer (< 4); Eptesicus fuscus, Idionycteris phyllotis and Myotis thysanodes (> 4.2). Prey hardness was positively correlated to minimum and mean temperatures, bat´s body weight, total and forearm lengths, cranial variables as: zygomatic breadth, mandibular length, height of the coronoid process, lower molar width, C-M3 superior and inferior rows length and upper molar width; and negatively to ultrasonic variables as total slope, call duration, low and high frequencies, band width and frequency maximum power. Considering phylogenies, prey hardness positively correlated to mandibular length, C-M3 inferior and superior rows lengths (p < 0.05). Our results showed that environmental, morphological and echolocation variables can be used as predictors of preferred insect prey in a community of vespertilionid bats.