Weak genotoxicity of acrylamide on premeiotic and somatic cells on the mouse

Abstract

The effects of acrylamide (AA) were evaluated, under the EEC/STEP project ‘Detection of Germ Cell Mutagens’, by carrying out several cytogenetic assays on mouse germ and somatic cells. The spermatid micronucleus (MN) test was applied after treatment of meiotically dividing or premeiotic S phase cells. Acute treatments (50 and 100 mg/kg i.p.) as well as subchronic exposure to AA (4 × 50 mg/kg, 4 i.p. injections at 24-h intervals) were performed. A weak increase of MN was induced only by treatment with AA of cells in S phase. Sister-chromatid exchange (SCE) analysis in differentiating spermatogonia treated i.p. with 50 and 100 mg/kg confirmed the weak genotoxicity of AA in the premeiotic stages of spermatogenesis. The application of the MN test in peripheral blood reticulocytes of the same animals used for the spermatid MN assay indicated that the cytogenetic effects induced by AA in the somatic and germ cell lines are comparable in magnitude. The results obtained in this study by applying the spermatid micronucleus assay are in very good agreement with those reported by two other laboratories with the same technique.