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Abstract
Centrioles are characterized by a nine-fold arrangement of microtubule triplets held together by an inner protein scaffold. These structurally robust organelles experience strenuous cellular processes such as cell division or ciliary beating while performing their function. However, the molecular mechanisms underlying the stability of microtubule triplets, as well as centriole architectural integrity remain poorly understood. Here, using ultrastructure expansion microscopy for nanoscale protein mapping, we reveal that POC16 and its human homolog WDR90 are components of the microtubule wall along the central core region of the centriole. We further found that WDR90 is an evolutionary microtubule associated protein. Finally, we demonstrate that WDR90 depletion impairs the localization of inner scaffold components, leading to centriole structural abnormalities in human cells. Altogether, this work highlights that WDR90 is an evolutionary conserved molecular player participating in centriole architecture integrity.
Highlights
IntroductionCentrioles and basal bodies (referred to as centrioles from here onwards for simplicity) are conserved organelles important for the formation of the centrosome as well as for templating cilia and flagella assembly (Bornens, 2012; Breslow and Holland, 2019; Conduit et al, 2015; Ishikawa and Marshall, 2011)
Centrioles and basal bodies are conserved organelles important for the formation of the centrosome as well as for templating cilia and flagella assembly (Bornens, 2012; Breslow and Holland, 2019; Conduit et al, 2015; Ishikawa and Marshall, 2011)
Our work demonstrates that POC16/WDR90 family proteins constitute an evolutionary conserved central core microtubule triplet component that is essential for maintaining the inner centriolar scaffold components in human centrioles
Summary
Centrioles and basal bodies (referred to as centrioles from here onwards for simplicity) are conserved organelles important for the formation of the centrosome as well as for templating cilia and flagella assembly (Bornens, 2012; Breslow and Holland, 2019; Conduit et al, 2015; Ishikawa and Marshall, 2011). Defects in centriole assembly, size, structure and number lead to abnormal mitosis or defective ciliogenesis and have been associated with several human pathologies such as ciliopathies and cancer (Gonczy, 2015; Nigg and Holland, 2018; Nigg and Raff, 2009). Centrioles are characterized by a nine-fold radial arrangement of microtubule triplets, are polarized along their long axis, and can be divided in three distinct regions termed proximal end, central core and distal tip (Hamel et al, 2017). Each region displays specific structural features such as the cartwheel on the proximal end, which is crucial for centriole assembly (Nakazawa et al, 2007; Strnad et al, 2007) or the distal appendages at the very distal region, essential for membrane docking during ciliogenesis (Tanos et al, 2013).
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