Ways to increase the immunogenicity of inactivated vaccines against strangles

Abstract

The results of the study on the specific prevention of infectious diseases in horses are presented. Methods have been developed to increase the immunogenicity of environmentally friendly vaccines against strangles, caused by the beta-hemolytic streptococcus – Sreptococcus equi. The work was carried out in laboratory conditions and horse breeding farms of the Republic of Sakha (Yakutia). The toxicity and immunogenicity of the vaccine was determined by conventional methods on young horses. Based on the study of the immunobiological reactivity of young horses, the use of immunomodulators in the development of inactivated vaccine preparations has been substantiated. Vaccines with immunomodulators were tested and registered in accordance with approved research methods for medicinal products for veterinary use. Polyribonate (polyvedrim) and culture liquid (fugate) from the bacterial strain Bacillus subtilis TNP-3 were used in the composition of vaccines as an immunomodulator. To prepare a vaccine against strangles, strains of bacteria Streptococcus equi H-34 and Streptococcus equi "H-5/1" were used, which were deposited in the Russian State Collection of Microorganism Strains of the Russian State Center for Animal Feed and Drug Standardization and Quality. After immunization with inactivated vaccines containing immunomodulators, the effectiveness of vaccines increases by 20% and reaches 90%. Vaccines increase the body's immunobiological reactivity. The most effective were the “Tabyn” vaccine and the vaccine from the Streptococcus equi strain H-5/1 with fugate, the Bacillus subtilis strain TNP-3. The polyribonate vaccine was approved in Russia (2000), the “Tabyn” vaccine is used in Kazakhstan (2018). These vaccine preparations, which ensure high antiepizootic efficacy, are environmentally friendly, since they do not contain toxic substances and antibiotics.