Abstract
A fast surface plasmon resonance (SPR) imaging biosensor system based on wavelength interrogation using an acousto-optic tunable filter (AOTF) and a white light laser is presented. The system combines the merits of a wide-dynamic detection range and high sensitivity offered by the spectral approach with multiplexed high-throughput data collection and a two-dimensional (2D) biosensor array. The key feature is the use of AOTF to realize wavelength scan from a white laser source and thus to achieve fast tracking of the SPR dip movement caused by target molecules binding to the sensor surface. Experimental results show that the system is capable of completing a SPR dip measurement within 0.35 s. To the best of our knowledge, this is the fastest time ever reported in the literature for imaging spectral interrogation. Based on a spectral window with a width of approximately 100 nm, a dynamic detection range and resolution of 4.63 × 10−2 refractive index unit (RIU) and 1.27 × 10−6 RIU achieved in a 2D-array sensor is reported here. The spectral SPR imaging sensor scheme has the capability of performing fast high-throughput detection of biomolecular interactions from 2D sensor arrays. The design has no mechanical moving parts, thus making the scheme completely solid-state.
Highlights
The surface plasmon resonance (SPR) sensing technique, which offers unique real-time and label-free measurement capabilities with high detection sensitivity, has become an important tool for exploring the kinetics of biomolecular interactions and has been widely used to detect chemical and biological analytes [1,2,3,4]
The SPR phenomenon produces a minimum in the reflectivity (SPR dip) when the incident p-polarized light resonantly couples with a surface plasma wave (SPW) at a specific angle or wavelength
Vector, np is the refractive index of the prism, λ is the wavelength of excitation light, εmr is the real part of a complex dielectric constant of Au, θ is the angle of incidence, and ns is the refractive index of the sample at the metal/dielectric interface [10,11]
Summary
The surface plasmon resonance (SPR) sensing technique, which offers unique real-time and label-free measurement capabilities with high detection sensitivity, has become an important tool for exploring the kinetics of biomolecular interactions and has been widely used to detect chemical and biological analytes [1,2,3,4]. Describe a fast spectral SPR imaging system (SPRi) based on an AOTF scanning3 of of9 the incident wavelength. Begin i min x array, a number of different resonant wavelengths are obtained from the sensor elements because each element has been designated to perform a specific sensing task.
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