Water-soluble polysaccharide from the fruiting bodies of Chroogomphis rutilus (Schaeff.: Fr.) O. K. Miller: Isolation, structural features and its scavenging effect on hydroxyl radical

Abstract

One water-soluble polysaccharide (CRP) was isolated from the fruiting bodies of Chroogomphis rutilus with a molecular weight ( M w) of 3.2 × 10 4 Da. According to partial acid hydrolysis, periodate oxidation and Smith degradation, methylation, FT–IR, GC–MS, and NMR analysis, the results indicated CRP had a backbone consisting of (1→6)-linked-α- d-galactopyranosyl and (1→2, 6)-linked-α- d-galactopyranosyl residues that terminated in a single terminal (1→)-β- d-glucopyranosyl residue at the O-2 position of (1→2, 6)-linked-α- d-galactopyranosyl residue along the main chain in the ratio of 1:1:1. Furthermore, the in vitro anti-oxidant activity evaluated by hydroxyl radicals scavenging method showed that CRP could remarkably enhance the scavenging effect on hydroxyl radicals in a dose-dependent manner.