Abstract
Glycoproteins were extracted with water from leaves of Cannabis sativa grown from seeds of Thailand origin. By ion exchange chromatography the material was separated into a neutral and an acidic fraction. Both glycoprotein fractions contained arabinose, galactose, glucose, mannose and xylose, and in addition rhamnose and galacturonic acid were present in the acidic fraction. The carbohydrate moieties were investigated by methylation analysis and Smith-degradation, whereas the glycopeptide linkage was studied by alkaline hydrolysis in the presence of NaBH 4 and Na 2SO 3, respectively. This linkage was shown to be of the serine- O-galactoside type. The carbohydrate structure is highly branched, the majority of branches terminating in arabinofuranose end groups. Arabinose is also present in the chain, predominantly (1 → 4)- and/or (1 → 5)-linked. Galactose makes up most of the main chain as (1 → 3)-linked residues but also constitutes end groups and branch points, as do mannose and/or glucose. Xylose and rhamnose are present as (1 → 4)- and (1 → 2)-linked units, respectively. Galacturonic acid is assumed to be (1 → 4)- linked with some branching at 3 position. The amino acid hydroxyproline, present in the glycoprotein of South African Cannabis leaves, was absent in the corresponding Thailand material.
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