Abstract
Photoacoustic imaging (PAI) is a hybrid non-invasive imaging technique used to merge high optical contrast and high acoustic resolution in deep tissue. PAI has been extensively developed by utilizing its advantages that include deep imaging depth, high resolution, and label-free imaging. As a representative implementation of PAI, photoacoustic microscopy (PAM) has been used in preclinical and clinical studies for its micron-scale spatial resolution capability with high optical absorption contrast. Several handheld and portable PAM systems have been developed that improve its applicability to several fields, making it versatile. In this study, we developed a laboratory-customized, two-axis, waterproof, galvanometer scanner-based handheld PAM (WP-GVS-HH-PAM), which provides an extended field of view (14.5 × 9 mm2) for wide-range imaging. The fully waterproof handheld probe enables free movement for imaging regardless of sample shape, and volume rate and scanning region are adjustable per experimental conditions. Results of WP-GVS-HH-PAM-based phantom and in vivo imaging of mouse tissues (ear, iris, and brain) confirm the feasibility and applicability of our system as an imaging modality for various biomedical applications.
Highlights
Photoacoustic imaging (PAI) is a label-free, non-invasive biomedical imaging technique that has been extensively studied and developed [1,2]
The optical absorption contrast of target biological tissues determines the intensity of the broadband US waves (i.e., PA waves), which are converted into analog electrical signals by US transducers [4]
We demonstrated the WP-GVS-HH-photoacoustic microscopy (PAM) with a laboratory customized two-axis WP-GVS that provides an extensive scanning range for a wide range in vivo 3D
Summary
Photoacoustic imaging (PAI) is a label-free, non-invasive biomedical imaging technique that has been extensively studied and developed [1,2]. PAI is based on the lightinduced ultrasound (US) signal through the photoacoustic (PA) effect (i.e., thermal-elastic expansion) [3]. Using the PA effect, PAI enables the non-invasive characterization of biological and biomedical properties with endogenous and exogenous agents such as metabolism, anatomy information, functional data, and molecular processes [1,6]. Based on the aforementioned distinctive advantages, PAI has been functionally used in various applications including vasculature mapping, assessing hemoglobin oxygen saturation, and blood flow [7,8,9,10]
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
