Abstract
In vivo monitoring of rice (Oryza sativa L.) seed germination and seedling growth under general conditions in closed Petri dishes containing agar base medium at room temperature (temperature = 24.5 ± 1 °C, relative humidity = 76 ± 7% (average ± standard deviation)), and induced degenerated callus formation with plant growth regulator, were performed using short-wavelength near-infrared spectroscopy and aquaphotomics over A period of 26 days. The results of spectral analysis suggest changes in water absorbances due to the production of common metabolites, as well as increases in biomass and the sizes of the samples. Quantitative models built to predict the day of the development provided better accuracy for rice seedlings growth compared to callus formation. Eight common water bands were identified as presenting prominent changes in the absorbance pattern. The water matrix of only rice seedlings showed three developmental stages: firstly expressing a predominantly weakly hydrogen-bonded state, then a more strongly hydrogen-bonded state, and then, again, a weakly hydrogen-bonded state at the end. In rice callus induction and proliferation, no similar change in water absorbance pattern was observed. The presented findings indicate the potential of aquaphotomics for the in vivo detection of degeneration in cell development.
Highlights
All living organs are made of cells, which are small membrane-bound compartments filled with a concentrated aqueous solution of metabolic components
Qualitative models revealed similarities in the main water absorbance bands activated in cell development, and differences in the spectral patterns and dynamics between the developmental process of callus and rice seedlings
principal component analysis (PCA) analysis revealed that absorbances at certain water absorbance bands and spectral regions can be related to the change in size of the samples, as well as to an increase in the number of cells
Summary
All living organs are made of cells, which are small membrane-bound compartments filled with a concentrated aqueous solution of metabolic components. Investigations of cell development are of importance for a better understanding of cell biology, but for practical applications as well. A number of biochemical and morphogenetic studies have been carried out to explore the basics of growth and development in plant cells. These studies have mainly been concerned with the detection of changes in concentrations of biochemical components, nucleic acid content, protein synthesis and/or other biomolecules [1,2,3,4]. The use of non-destructive spectroscopy methods for in vivo investigation, without any disruption of the system dynamics, could lead to a better understanding of the complexity of the cell development process. Apart from bringing new insights to developmental biology, this provides a new non-invasive tool for growth and development monitoring and control
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
