Water-soluble organic materials in paddy soil ecosystem

Abstract

Abstract Column experiments were conducted to analyze the effect of the temperature on the amounts of organic materials in the leachate, especially organic acids and methane, from samples of the plow layer soil amended with rice straw. Total amount of inorganic carbon in the leachate during the 30-d period of incubation in relation to the temperature was 18°C < 25°C ≤ 30°C > 37°C > 45°C. Total amount of organic carbon in the leachate was signiicantly larger under 45°C incubation than that at other temperatures. Acetic acid was the dominant organic acid in the leachate regardless of the temperature. Butylic and propionic acids were also present in large amounts in the early and the late period of incubation of temperatures ranging between 18 and 37°C, while only acetic acid was the dominant organic acid during the 30-d period of incubation at 45°C. The total amount of methane in leachate during the 30-d period of incubation was very small at 18°C, while very large at 25, 30, and 37°C. It decreased nearly to one half at 45°C compared with that at 30°C. Based on the values of δ13CH4 in the leachate, 3 different stages were recognized in the predominant processes of methane production in the submerged paddy soil amended with rice straw: the stage when methane production from CO2-B2 was predominant followed by the stages of methane production from acetic acid and from CO2-H2 in this order. The second stage coincided with the time of decrease of the organic acid contents in the leachate. Under 45°C incubation, methane production from CO2-H2 was predominant throughout the 30-d period of incubation.