Water replacement to reduce tetracycline residue level in the liver of tilapia (Oreochromis sp.)

Abstract

Tetracycline is an antibiotic that frequently used to treat various bacterial infections. However, the residue of the antibiotic are often found in food of animal origin, including fish and can be hazardous if consumed by human. This study aimed to determine the effect of water replacement on the residual levels of tetracycline in the liver of tilapia after therapy. Fifty four tilapia (Oreochromis sp.) were divided into 3 groups, namely group A, B, and C. Group A as a negative control was injected with 0.25 ml NaCl 0.9% without water replacement, group B as a positive control was injected with 0.25 ml tetracycline (50 mg/kg) without water replacement, and group C was injected with 0.25 ml tetracycline (50 mg/kg) with 25% water replacement twice a day for 6 days. Subsequently, 3 tilapias were euthanized from each group daily with cranial concussion. Samples of the liver were taken. Liver were analyzed by HPLC for tetracycline residual level and analyzed with Two Way ANOVA. The results showed that there were significant differences in residual level between groups and day. It can be concluded that 25% water replacement can decrease residual level in the tilapian liver.