Abstract
The effect of water activity ( a w ) upon glycerol production during ethanol fermentation by Saccharomyces cerevisiae was studied in continuous culture using sorbitol to adjust the a w to 0.971. A reduction of the a w of the medium resulted in more glycerol being produced. The glycerol yield was 3–4-fold greater at 0.971 a w than 0.994 a w . Ethanol yield and specific rate of ethanol production was slightly greater at 0.971 than 0.994 a w whereas the biomass concentration was lower. Reduced a w increased the specific rate of glucose uptake and the maintenance requirements of the organism. The results suggest that the effect of a w on glycerol production can be reduced by controlled feeding of substrate during fermentation.
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