Water-plasma-enhanced and phase-separation-assisted extraction of microalgal lipid for biodiesel production

Abstract

Traditional methods for lipid extraction from microalgal biomass usually involve harsh reaction conditions or the use of contaminant reagents, which lead to enormous energy consumption and wastage. Hence, a novel strategy was presented, which combined water-plasma and three-phase partitioning (TPP) techniques. Benefiting from its unique advantages such as rapid and low cost, water-plasma strategy can disrupt microalgal cell wall and can thus greatly affect lipid extraction. As a result, assisted with the TPP method, excellent performance lipid recovery (74.34%) was obtained at 200 W in 10 min. The performance was superior to that achieved through cell disruption via water-plasma pretreatment. Importantly, the whole process of lipid extraction prevented the drying of microalgal biomass, contributing to reduced energy consumption in large-scale biodiesel production. Moreover, the high fatty acids content suggested that the extracted lipids are great potential candidate for biodiesel production.