Abstract
Industrial hemp (Cannabis sativa) is traditionally cultivated as a valuable source of fibers and nutrients. Multiple studies also demonstrated antimicrobial, anti-proliferative, phytotoxic and insecticide effects of the essential oil from hemp female inflorescences. On the other side, only a few studies explored the potential pharmacological application of polar extracts from inflorescences. In the present study, we investigated the water extract from inflorescences of industrial hemp Futura 75 variety, from phytochemical and pharmacological point of view. The water extract was assayed for phenolic compound content, radical scavenger/reducing, chelating and anti-tyrosinase effects. Through an ex vivo model of toxicity induced by lipopolysaccharide (LPS) on isolated rat colon and liver, we explored the extract effects on serotonin, dopamine and kynurenine pathways and the production of prostaglandin (PG)E2. Anti-proliferative effects were also evaluated against human colon cancer HCT116 cell line. Additionally, antimycotic effects were investigated against Trichophyton rubrum, Trichophyton interdigitale, Microsporum gypseum. Finally, in silico studies, including bioinformatics, network pharmacology and docking approaches were conducted in order to predict the putative targets underlying the observed pharmacological and microbiological effects. Futura 75 water extract was able to blunt LPS-induced reduction of serotonin and increase of dopamine and kynurenine turnover, in rat colon. Additionally, the reduction of PGE2 levels was observed in both colon and liver specimens, as well. The extract inhibited the HCT116 cell viability, the growth of T. rubrum and T. interdigitale and the activity of tyrosinase, in vitro, whereas in silico studies highlighting the inhibitions of cyclooxygenase-1 (induced by carvacrol), carbonic anhydrase IX (induced by chlorogenic acid and gallic acid) and lanosterol 14-α-demethylase (induced by rutin) further support the observed pharmacological and antimycotic effects. The present findings suggest female inflorescences from industrial hemp as high quality by-products, thus representing promising sources of nutraceuticals and cosmeceuticals against inflammatory and infectious diseases.
Highlights
Industrial hemp (Cannabis sativa) has been long extensively cultivated throughout history as a valuable source of fibers and nutrients [1]
The water hemp extract was formerly analyzed from a phytochemical point of view
Presentwas study, we furtheragainst explored the effectsproduction of hemp extract metabolism, taking into we further explored the effects of hemp extract on metabolism, taking into consideration consideration the level of 5-HIIA, the main 5-HT metabolite and the levels of KA and 3-HK, thatthe are level of 5-HIIA, the main of metabolite and KYN
Summary
Industrial hemp (Cannabis sativa) has been long extensively cultivated throughout history as a valuable source of fibers and nutrients [1]. The hemp chain production was principally focused on fiber production and seed-deriving foods, in the last years there has been a renewed interest in the study and valorization of hemp-deriving extracts, that are sources of terpenes, terpenophenolics, amino acids, fatty acids, sugars, hydrocarbons, flavonoids, with promising health-promoting and pharmacological effects [5] In this regard, multiple studies focused on the antimicrobial, anti-proliferative, phytotoxic and insecticide effects of the essential oil from hemp female inflorescences [6,7,8], sold dried for technical use and usually considered as a waste material of industrial hemp chain production [9]. Taking into consideration the inhibitory effects induced by this extract on both Candida albicans and C. tropicalis [10], we explored the antimycotic effects of Futura 75 water extract on multiple dermatophytes species, namely Trichophyton rubrum, Trichophyton interdigital, Microsporum gypseum
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
