Water and ion balance in hydrating oocytes of the grey mullet, Mugil cephalus (L.), during hormone‐induced final maturation

Abstract

Sequential changes in water content and inorganic ion concentrations occurring in intra‐ovarian oocytes of the grey mullet, Mugil cephalus L., during hormone‐induced final maturation are described. Post‐vitellogenic oocytes have a water content of 59.4%. During final maturation, oocyte water increases rapidly to a maximum of 84.8% at oviposition. Hydration is accompanied by a relatively small increase in dry matter due to the concurrent uptake of inorganic ions and an apparent uptake of organic matter.Net uptake of Ca2+ during hydration is negligible. Net uptake of Mg2 + and Na+ occurs in less than equimolar proportions. Net uptake of K+ and Cl− occurs in greater than equimolar proportions prior to ovulation, returning to equimolarity at oviposition. Hence, hydration results in a dilution of intracellular Ca2+, Mg2+ and Na+ to minimum concentrations at oviposition. In contrast, K+ and Cl− concentrations are slightly elevated prior to ovulation, returning to initial levels at oviposition. A 1:1 stoichiometric relationship in net uptake of K + andCl− is evident.Intracellular osmotic pressure in post‐vitellogenic oocytes is 203.4 ± 18.0 mOsmolkg−1. Osmotic pressure rises to a peak of 421.3+ 17.3 mOsmol kg−1 prior to ovulation, then declines to 313.3 ± 15.0 mOsmol kg−1 at oviposition. This rise in osmotic pressure prior to ovulation cannot be accounted for solely on the basis of inorganic ion uptake and must be attributed to changes in intracellular concentrations of organic solutes as well. An apparent increase in non‐ash dry matter during hydration suggests that a net uptake of organic solute is involved. A relatively small organic molecule with high osmotic activity (e.g., amino acids) is implicated.