Abstract
Visualization of biomolecules in live cells plays a major role in advancing the field of life sciences. To this end, many bioimaging tools have been developed, among which fluorescence microscopy is most widely used. However, fluorescence labeling has problems with imaging small molecules because the larger size of the label often perturbs the biological activity and localization of the target small molecule. To detect small bioactive molecules, we use a small chemical tag called alkyne and detect its unique vibrational signature by Raman microscopy.
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