WAPO-A1 is the causal gene of the 7AL QTL for spikelet number per spike in wheat.

Saarah Kuzay,Junli Zhang,Daniel P Woods,Shisheng Chen,Chengxia Li,Huiqiong Lin,Jorge Dubcovsky,Tianyu Lan,Maria Von Korff,Sarah Hake

doi:10.1371/journal.pgen.1009747

Abstract

Improving our understanding of the genes regulating grain yield can contribute to the development of more productive wheat varieties. Previously, a highly significant QTL affecting spikelet number per spike (SNS), grain number per spike (GNS) and grain yield was detected on chromosome arm 7AL in multiple genome-wide association studies. Using a high-resolution genetic map, we established that the A-genome homeolog of WHEAT ORTHOLOG OF APO1 (WAPO-A1) was a leading candidate gene for this QTL. Using mutants and transgenic plants, we demonstrate in this study that WAPO-A1 is the causal gene underpinning this QTL. Loss-of-function mutants wapo-A1 and wapo-B1 showed reduced SNS in tetraploid wheat, and the effect was exacerbated in wapo1 combining both mutations. By contrast, spikes of transgenic wheat plants carrying extra copies of WAPO-A1 driven by its native promoter had higher SNS, a more compact spike apical region and a smaller terminal spikelet than the wild type. Taken together, these results indicate that WAPO1 affects SNS by regulating the timing of terminal spikelet formation. Both transgenic and wapo1 mutant plants showed a wide range of floral abnormalities, indicating additional roles of WAPO1 on wheat floral development. Previously, we found three widespread haplotypes in the QTL region (H1, H2 and H3), each associated with particular WAPO-A1 alleles. Results from this and our previous study show that the WAPO-A1 allele in the H1 haplotype (115-bp deletion in the promoter) is expressed at significantly lower levels in the developing spikes than the alleles in the H2 and H3 haplotypes, resulting in reduced SNS. Field experiments also showed that the H2 haplotype is associated with the strongest effects in increasing SNS and GNS (H2>H3>H1). The H2 haplotype is already present in most modern common wheat varieties but is rare in durum wheat, where it might be particularly useful to improve grain yield.

Highlights

Wheat is an essential staple crop for global food security
We show that loss-of-function mutations in the WHEAT ORTHOLOG of APO1 (WAPO1) gene located within this region reduce the number of spikelets per spike and that additional transgenic copies of this gene increase this number
These results demonstrate that WAPO1 is the gene responsible for the differences in grain number and yield associated with the 7A chromosome region

Summary

Introduction

Wheat is an essential staple crop for global food security. It is highly adapted to a wide variety of climates and production systems, and provides more than 20% of the calories and protein consumed by the human population [1]. Further increases in grain yield are required to feed a continuously growing population, historical yield trend studies have shown a decrease in the relative rates of grain yield gains in some wheat growing regions [2]. This has prompted new efforts to understand and improve the productivity of both common (Triticum aestivum, genomes AABBDD) and durum wheat SNS usually exhibits high heritability because it is established early in the reproductive phase when the terminal spikelet is formed [4], limiting the effect of environmental conditions after this point

Methods

Results

Discussion

Conclusion