Abstract
Wait and capture: unwinding the strategy of a DEAD-box helicase.
Highlights
RNA plays a wide variety of roles in every cell, from structural to informational to catalytic
While much has been learned about these proteins, several important details of their mechanism of action remain unknown. It has not been clear what role, if any, the helicase plays in undoing the tertiary contacts made between the target helix and other parts of the RNA molecule. In this issue of PLOS Biology, Cynthia Pan, Rick Russell, and colleagues demonstrate that one such helicase waits for those tertiary contacts to detach before capturing the helix, preventing rebinding and preparing it for unwinding
Two different dyes are attached to each member of an interacting pair of molecules, in this case the ribozyme and its oligonucleotide substrate, which binds to the ribozyme to form the P1 helix
Summary
RNA plays a wide variety of roles in every cell, from structural to informational to catalytic. Wait and Capture: Unwinding the Strategy of a DEADBox Helicase Among these chaperones are the socalled DEAD-box helicases, which bind to short double-helical sections of RNA, and, powered by ATP, unwind them.
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