Wachstum und N‐Stoffwechsel sowie Anderungen des Isoenzymspektrums der Glutamatdehydrogenase (GDH) in batchvermehrten, licht‐ und dunkelkultivierten Zellsuspensionen von Pisum sativum

Abstract

Abstract Growth, N‐metabolism and isoenzyme pattern of glutamate dehydrogenase in batch‐cultures of Pisum sativum cells under light and dark conditions.Cell suspension cultures of Pisum sativum L. derived from root and shoot sections of seedlings have been prepared and cultured in defined nutrient medium. Both the cells and the media were analysed daily for the N‐fractions and carbohydrates during the growth period.The data obtained indicate specific correlations between growth and nitrogen and carbohydrate metabolism. At the beginning of the growth cycle ammonia as compared to nitrate was favoured in uptake. An increased uptake of nitrate occurred at the end of the linear growth phase when carbohydrate in the media was depleted. The uptake of sucrose was rapid during the whole growth cycle, only in the range of the linear growth phase the uptake stagnated for 3 or 4 days. During increased biosynthesis of nitrogenous compounds at the beginning of the growth cycle up to seven isoenzymes of the glutamate dehydrogenase could be separated by polyacrylamide gel electrophoresis. The isoenzyme pattern changed during the stationary growth phase, especially when the carbohydrate content in the medium decreased. There is some evidence that the isoenzyme pattern is influenced by carbohydrate metabolism.