W1365 Axial Stretch Activated Lower Esophageal Sphincter Relaxation Is Caused By the Direct Activation of Inhibitory Motor Neuron in the Myenteric Plexus

Abstract

increase in the branching pattern of isolated rat plexus neurons compared to control (chemically defined medium). Regarding the effects on fiber density of DRG neurons, 75% of all extracts induced an increase. Neurotrophin measurements showed an enormous variation within the different individual samples, decreasing from ganglionic to aganglionic in 50 % of the samples, while all other were either increasing or stable. So although deriving from the same disease, the individual samples displayed a huge variation in their microenvironmental conditions. But nevertheless we could clearly show in the presented study that gut muscle extracts from HSD patients did not have a negative effect on isolated rat plexus or DRG neurons In Vitro. This result is of particular importance, because it is a valuable evidence that the aganglionic bowel does provide a satisfactory microenvironment for the transplantation of neuronal stem cell as a future therapy for HSD.