Abstract
INTRODUCTION: We investigate a completely xenogeneic-free expansion method for adipose-derived stem cells (hADSC) to maintain their properties and enhancing their neurotrophic potential to develop a reliable and safe strategy to be extended to nerve regeneration in vivo. MATERIALS AND METHODS: We compared isolation methods and supplement media (fetal bovine serum (FBS) or human-platelet lysate (hPL)) on hADSC morphology, proliferation rate, immunophenotype, lineage differentiation potential and neurogenic commitment (nerve growth factors secretion). Functional analysis of hADSC expanded in both medium conditions was performed using an in vitro co-culture model with rat dorsal root ganglia (DRG) explants, following neurite outgrowth. RESULTS: We found no significant difference between the means of cell isolation and the supplements except for a higher proliferation rate and more elongated morphology of hPL-hADSC. Neurotrophic factor secretion by hPL-hADSC showed statistically higher levels in all three growth factors compared with FBS-hADSC. DRG showed significantly longer neurite length and higher axonal area when co-cultured with hPL-hADSC. CONCLUSION: We show that hPL provides a clinically translatable means to support hADSC, strengthening cells proliferation and further promoting their neurotrophic properties in vitro.
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