Vp1 expression profiles during kernel development in six genotypes of wheat, triticale and rye

Abstract

During the last few decades, the physiological and genetic background of dormancy, and correlated pre-harvest sprouting (PHS) have been intensively investigated. Special attention has often been paid to genetic factors that may explain and predict PHS susceptible behaviour. A major candidate is the Vp1 gene which is involved in embryo development and maturation as well as in dormancy establishment. In this study, Vp1 gene expression during kernel development was studied in wheat, triticale and rye as a potential biomarker for selecting PHS tolerant varieties in cereal breeding programs. Plants of known PHS tolerant and PHS susceptible varieties were grown under controlled conditions from flowering until harvest ripeness. During that period, kernels were regularly harvested for RNA extraction and cDNA synthesis. Calibrated and normalized relative Vp1 expression levels were obtained in an RT-qPCR assay. During kernel development, Vp1 expression levels generally showed a typical peak during the soft dough stage, after which they decreased and remained low until harvest maturity. Differences in Vp1 expression levels could be observed between the PHS susceptible and PHS tolerant varieties of wheat, with the PHS tolerant variety showing higher levels of relative Vp1 expression compared to the PHS susceptible variety. In triticale, however, this difference was only seen once and could not be confirmed in further experiments. It seems that the Vp1 gene in triticale behaves in a similar way as in rye, in which no specific trends could be observed.