インフルエンザウイルスVon Magnus型不完全粒子の構造に関する研究

Abstract

The morphological characteristics of “Standard Passage” virus and “Undiluted Passage” virus prepared for electron microscopy by the usual air-drying and by freeze-drying were compared with each other, and the densities of these viruses were also compared. The results were obtained as follows:1. SP virus isolated from allantoic fluids of infected eggs wee found to contain uniform particles of predominant by spherical shape with smooth surface, and as far as the appearence of particles was concerned, there was no great difference between an air-dried specimen and a frozen-dried specimen.2. In contrast, UP virus obtained from allantoic fluid of infected eggs vas found to be pleomorphic and there were great differences between an air-dried specimen and a frozen-dried specimen. That is to say, in the air-dried preparation, the majority of the particles appeared flattened and doughnuts like, but in the frozen-dried preparation, the majority of the particles appeared appromimately spherical in form and flatted or doughnut like particles disappeared.3. The density of the SF virus was estimated at 1.10 in blood albumin solution anti 1.23 in saline solution, meanwhile, the density of the UP virus was estimated at 1.07 in blood albumin solution and 1.16 in saline solution.From these results, the existence of basic structural differences between the two types of virus was estimated.