Volumetric power consumption in baffled shake flasks

Abstract

For the cultivation of microorganisms, baffled shake flasks are employed when increased levels of oxygenation and mixing are required. Their use has been discouraged, however, due to the danger of a wetted sterile plug and the lower reproducibility of the experimental results. Consequently, there are only few studies dealing with this type of shaken bioreactor, and there is practically no characterization of this reactor type from a chemical engineering viewpoint. Therefore, a systematic study to elaborate the basic characteristics of the volumetric power consumption and the unfavorable out-of-phase phenomenon in baffled shake flasks is undertaken. A new type of measuring device was developed to measure the volumetric power consumption in a single shake flask. The volumetric power consumption was found to increase with increasing shaking frequency and with decreasing filling volume. Further, an independency of power consumption on the shaking diameter was observed as long as the fluid motion is in-phase. A comparison of two different baffle geometries demonstrated that deeper baffles cause more resistance to fluid flow. For the commonly employed shaking diameter of 25 mm, the investigated baffled flask types may not be operated in the in-phase state. A larger shaking diameter must therefore be employed. It was found for the first time that for all in-phase conditions, the dimensionless Newton number Ne ′ is independent of the Reynolds number Re. Power consumption in baffled shake flasks may therefore be described by a characteristic Ne ′ only dependent on the filling volume V L and the flask type. Even though there are quantitative differences, a qualitative similarity between fluid flow in stirred tanks and shake flasks has been demonstrated.