Abstract
Phase variance-based motion contrast imaging is demonstrated using a spectral domain optical coherence tomography system for the in vivo human retina. This contrast technique spatially identifies locations of motion within the retina primarily associated with vasculature. Histogram-based noise analysis of the motion contrast images was used to reduce the motion noise created by transverse eye motion. En face summation images created from the 3D motion contrast data are presented with segmentation of selected retinal layers to provide non-invasive vascular visualization comparable to currently used invasive angiographic imaging. This motion contrast technique has demonstrated the ability to visualize resolution-limited vasculature independent of vessel orientation and flow velocity.
Highlights
Fluorescein angiography is the gold standard for vascular imaging of the retina
Phase variance-based motion contrast imaging is demonstrated using a spectral domain optical coherence tomography system for the in vivo human retina. This contrast technique spatially identifies locations of motion within the retina primarily associated with vasculature
Histogrambased noise analysis of the motion contrast images was used to reduce the motion noise created by transverse eye motion
Summary
Fluorescein angiography is the gold standard for vascular imaging of the retina. Its improved variation called spectral or Fourier domain optical coherence tomography (FD-OCT) [5,6], due to improved sensitivity and acquisition time [7], allowed for volumetric retinal imaging in clinical setting [8,9] Functionality improvements such as Doppler OCT and similar methods enable OCT to visualize regions of flow in the retina, but visualization has typically been limited to the major veins and arteries within the eye [10,11,12,13,14]. We have developed a novel motion contrast technique for FD-OCT capable of visualizing a wider range of velocities and flow orientations than Doppler OCT techniques This method has been demonstrated for in vivo by visualization of vasculature in the zebrafish and the retinal and choroidal vasculature of the mouse [15,16]. In this paper we present the initial results of adapting this motion contrast technique to normal human retinal OCT imaging, demonstrating the microvascular imaging capabilities for multiple layers within the retina
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