Abstract
Measles virus has no or indistinctive cytopathic effects (CPE) in cell couture system. Employment of some detecting methods like plaque assay or stereologic experiments, as a method of detecting of viral infection in the cells would be applicable. The aim of this study was investigating the early changes in quantitative parameters of measles virus infected Vero cells. Stereological methods using invariator, were applied for the first time to estimate cell and nucleus volume and cell surface of the infected Vero cell line with the measles virus.This method can be applied on other cultured cells.Vero cells grown in tissue culture plates for 48 hours at 36˚C were infected with 100TCID50 of AiK strain of measles virus. Volume and surface of the infected Vero cells were studied at 4, 9 and 25 hours post infection along with uninfected control cells. The mean cell volume and surface of the cells infected with measles virus, increased ~87% and ~50%, respectively, 4 hours post-infection, as compared with the uninfected control. The nuclei did not show any differences. The mean parameters of infected cells in other time intervals showed no significant difference comparing with the control cells. Although there are other specific methods, stereology may be used as an integrated protocol to detect cytophatic changes of the measles virus infected cells early in the permissive cell culture system.
Highlights
Cell culturesThe Vero cell culture obtained from the Cell Bank of Pasteur Institute, Tehran, Iran, was grown in Dulbecco Minimal Essential Medium (DMEM), supplemented with 5% fetal bovine serum (FBS) from GIBCO
Typical measles is diagnosed on clinical grounds, its laboratory diagnosis is necessary in modified or atypical cases
Stereological methods using invariator, c were applied for the first time to estimate cell r and nucleus volume and cell surface of the e infected Vero cell line with the measles virus.This method can be applied on other culm tured cells.Vero cells grown in tissue culture plates for 48 hours at 36°C were infected with m 100TCID50 of AiK strain of measles virus. o Volume and surface of the infected Vero cells c were studied at 4, 9 and 25 hours post infection - along with uninfected control cells
Summary
The Vero cell culture obtained from the Cell Bank of Pasteur Institute, Tehran, Iran, was grown in Dulbecco Minimal Essential Medium (DMEM), supplemented with 5% fetal bovine serum (FBS) from GIBCO. All media contained 100 IU/mL of penicillin and 100 μg/mL of streptomycin sulphate
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