Voltammetric Sensing of Soybean Agglutinin Using an Electrode Modified with Electron‐transfer, Carbohydrate‐mimetic/Cross‐linker‐peptide‐collagen Film

Abstract

AbstractWe constructed an electrochemical sensor based on an electrode modified with electron‐transfer, carbohydrate‐mimetic peptides on collagen film. The peptide consisted of Ac‐Y4C combined with soybean agglutinin (SBA). To evaluate the binding between SBA and the peptide, we prepared Ac‐Y4CAn (3‐6) containing oligoalanine as a cross‐linker. When SBA and Y4C on the electrode were incubated, the peak was decreased by the SBA uptake of the peptide. The change in the peak current using Ac‐Y4CA6 was the greatest of the four peptides. The calibration curve was linear and ranged from 4.0×10−14 to 1.2×10−12 M with a detection limit of 1.3×10−14 M.