Voltammetric determination of propranolol enantiomers in the model solutions of pharmaceutical forms and biological fluids

Abstract

The possibilities of new voltammetric sensors based on glassy carbon electrodes modified by the composites of polyarylenephthalide with melamine, cyanuric acid, α- and β-cyclodextrins for the determination of propranolol enantiomers in the model solutions of pharmaceutical and biological fluids were studied. The electrochemical and analytical characteristics of voltammograms of propranolol enantiomers and their pharmaceutical dosage forms on the modified electrodes were obtained. The voltamperograms of the propranolol enantiomers on these electrodes differ among themselves both in the values of the instantaneous currents and in the potentials of oxidation. The proposed sensors allowed determining the propranolol enantiomers in a racemic mixture and in a mixture with the predominance of one of the enantiomers. The dependence of the analytical signal on the concentration of propranolol was linear in the range from 0.021 to 0.675 mM, the detection limit was (5.28÷8.37)×10 -6 М in the solutions of enantiomers and (2.75÷5.57)×10 -5 М in pharmaceutical dosage forms respectively. The relative standard deviation for the pure solutions of propranolol enantiomers ranged from 0.011 to 0.064, and for pharmaceutical dosage forms of propranolol it ranged from 0.022 to 0.109. The auxiliary substances did not have a significant interfering effect. The developed enantioselective sensors were used for the determination of propranolol enantiomers in the biological fluids. The statistical evaluation of the results by the "spiked -found" method indicated that there was no significant systematic error. The relative standard deviation in the determination of propranolol enantiomers ranged from 0.071 to 0.109 in the urine, and from 0.116 to 0.146 in the plasma. Keywords : enantiomers, propranolol, voltammetry, polyarylenephthalide, melamine, cyanuric acid, cyclodextrins, composite electrodes, auxiliary substances, biological fluids DOI: http://dx.doi.org/10.15826/analitika.2018.22.3.012 (Russian) R.A. Zilberg, Yu.A. Yarkaeva, Yu.R. Provorova, V.Yu. Gus’kov, V.N. Maistrenko Bashkir State University, Zaki Validi str. 32, Ufa, 450076, Russian Federation