Voltammetric determination of glycopyrrolate in a pharmaceutical formulation

Muneer Fadr,Samer Ratrout,Abdulaziz Nabil Amro,Sami Ben Aoun,Fadi Asfour

doi:10.3906/kim-1801-37

Abstract

The electrochemical activity of glycopyrrolate was studied. Different voltammetric techniques were applied in this study, including cyclic voltammetry (CV), differential pulse voltammetry (DPV), and linear sweep voltammetry (LSV). Glassy carbon and platinum working electrodes were utilized. CV indicated that glycopyrrolate has a reversible redox reaction on the working electrode surface, with an anodic peak current at approximately 1.05 V and a cathodic peak at 0.80 V. Several parameters that affect the sensitivity of these methods were optimized for the quantitation of glycopyrrolate. LSV showed a better correlation coefficient than DPV did, with a value of ca. 0.9990 in the range of 0.1-0.5 mg/mL. The LSV and DPV recovery and relative standard deviation (RSD) results fell within the accepted range, with a better recovery (102.07%) for DPV and a better RSD (0.511%) for LSV. The limits of detections were ca. 16 and ca. 25 μg/mL for LSV and DPV, respectively.

Highlights

The most common instrumental analysis methods used for quantitation in pharmaceutical analysis are chromatography, spectrophotometry, and electrochemistry.[1]
The aim of this work is the determination of glycopyrrolate in pharmaceutical preparations using different voltammetric techniques, namely linear sweep voltammetry (LSV), cyclic voltammetry (CV), and differential pulse voltammetry (DPV)
Optimization of working electrodes and supporting electrolytes The CV method was used to study the electroactivity of glycopyrrolate

Summary

Introduction

The most common instrumental analysis methods used for quantitation in pharmaceutical analysis are chromatography, spectrophotometry, and electrochemistry.[1]. 2,3 The standard techniques that are currently used for the determination of drugs in biological fluids, bulk form, and pharmaceutical formulations are based on chromatographic or spectroscopic assays.[2] Such techniques for the determination of drug concentrations are necessary in a clinical environment to provide appropriate drug levels and avoid toxic concentrations of these drugs. 2 The high costs of the instrumentation and operation of spectroscopic and chromatographic techniques make electrochemical techniques, which are simpler, faster, cheaper, and more sensitive, better alternatives. Glycopyrrolate is a synthetic anticholinergic agent with a quaternary ammonium structure.

Objectives

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Results